Figure 4 | Scientific Reports

Figure 4

From: Oriented immobilization of basic fibroblast growth factor: Bioengineered surface design for the expansion of human mesenchymal stromal cells

Figure 4

The results of adhesion and proliferation assays on the bFGF-His-7 immobilized surfaces prepared using SAMs with different alkanethiol compositions. (A–F) Phase contrast images of hMSCs 24 h after cell seeding. A COOH-thiol content in the SAM: (A) 0%, (B) 20%, (C) 40%, (D) 60%, (E) 80%, and (F) 100%. Scale bar: 200 µm. (G) The result of hMSC proliferation assays plotted as a function of COOH-thiol contents. The assays were conducted 6 day after cell seeding. Data are expressed as the mean ± standard deviation for n = 4. Asterisks indicate significance difference compared to the case with 100% COOH-thiol (Student’s t-test, p < 0.05).

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