Figure 6

Secondary structural changes of immobilized bFGF-His-7 during the in situ treatment with citrate buffer. (A) Far-ultraviolet CD spectra of immobilized bFGF-His-7 after the citrate buffer treatment for (black) 0, (purple) 5, (red) 10, (green) 30, (orange) 60, and (yellow) 120 min. (B) Mean residue molar ellipticity at (open circle) 208 and (closed circle) 216 nm as a function of the duration of the citrate buffer treatment.