Figure 7 | Scientific Reports

Figure 7

From: Oriented immobilization of basic fibroblast growth factor: Bioengineered surface design for the expansion of human mesenchymal stromal cells

Figure 7

The proliferation of hMSCs on the bFGF-His-immobilized, in situ citrate buffer-treated surface. (A) Phase contrast images of hMSCs cultured on the surface of (left) the bFGF-His-immobilized, in situ citrate buffer-treated substrates and (right) polystyrene plates for (upper) 1 day and (bottom) 6 days. Scale bar: 200 μm. (B) The results of hMSCs proliferation assays on the surface of (open circle) the bFGF-His-immobilized, in situ citrate buffer-treated substrates and (closed circle) polystyrene plates. Data are expressed as the mean ± standard deviation for n = 4. Student’s t-test was performed to analyze significance in difference between the two conditions at the same time points (*p < 0.05).

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