Figure 8 | Scientific Reports

Figure 8

From: Oriented immobilization of basic fibroblast growth factor: Bioengineered surface design for the expansion of human mesenchymal stromal cells

Figure 8

The results of differentiation assays with (A–C) hMSCs obtained from the bFGF-His-immobilized, in situ citrate buffer-treated substrates and (D–F) polystyrene plates. (A,D) Optical microscopic images of cells cultured in the osteogenic medium for 28 days and then stained with Alizarin red. (B,E) Optical microscopic images of cells cultured as pellets in the chondrogenic medium for 28 days and then stained with Alcian blue. (C,F) Optical microscopic images of cells cultured in the adipogenic medium for 21 days and stained with oil red O. Scale bar: 200 μm.

Back to article page