Figure 1

(a) Schematic representation of DENV particle showing the viral genome (blue), the capsid protein (red), and the lipid membrane containing the envelope (E) and pro-membrane (prM) proteins. (b) Representation of the DENV virus genome (DENV wild type) and the recombinant DENV carrying the capsid sequence fused to mCherry, followed by the food and mouth disease virus 2 A auto cleavage site. The complete sequence of the viral genome is indicated as prM E Non Structural region. (c) Protocol of viral RNA transfection. (d) Immunofluorescence using anti-C antibodies (DENV wild type) and C-mCherry visualization (DENV C-mCherry), both 24 h post-RNA transfection. (e) Immunofluorescence using anti-C antibodies (DENV wild type), C-mCherry visualization (DENV C-mCherry), and lipid droplets staining with BODIPY, both 24 h post-RNA transfection.