Figure 3

RICS measurement of C-mCherry diffusion. (a) Representative confocal image of a BHK cell transfected with C-mCherry (4 hours post transfection). A ROI of 256 × 256 pixels (12.8 × 12.8 μm²) is selected for RICS analysis in nucleus and cytoplasm. (b) Representation of image shifting for RICS analysis for the nucleus and (c) the cytoplasm. (d) Obtained fitted function of an image correlation result in a ROI after averaging 100 consecutive frames (top) and corresponding residual plot (bottom). Pixel shift starting at 0 is plotted from the center. (e) Effective diffusion coefficients obtained from the autocorrelation function of C-mCherry motion in nucleus and cytoplasm from 3 to 6 hours post transfection. Each bar represents a measurement in a single cell. 40 measurements from at least 5 independent cell cultures are shown.