Figure 3 | Scientific Reports

Figure 3

From: Extracellular vesicles from genetically unstable, oncogene-driven cancer cells trigger micronuclei formation in endothelial cells

Figure 3

Extracellular vesicle (EV)-mediated emission of genomic DNA sequences from RAS-3 cells is sensitive to autophagy inhibitor chloroquine. (A) Filtration/ultracentrifugation protocol to separate putative carriers of extracellular DNA (apoptotic bodies, microvesicles, small vesicles, soluble DNA). (B) HRAS DNA copy number per microliter of conditioned media in fractions (defined in panel A). (C) Lysotracker staining indicates retention of the dye in lysosomes of cells treated with chloroquine (inhibition of autophagy). (D) Chloroquine reduces the content of HRAS DNA in the EV fraction of RAS-3 conditioned media; ***p < 0.001.

Back to article page