Figure 5 | Scientific Reports

Figure 5

From: Lentiviral mediated RPE65 gene transfer in healthy hiPSCs-derived retinal pigment epithelial cells markedly increased RPE65 mRNA, but modestly protein level

Figure 5

GFP reporter gene expressions by lentiviral vector transduction reveals iRPE cells specificity. (a) Six different lentiviral vector constructs were produced bearing a GFP under ubiquitous (EFS), RPE specific (R0.8 = partial RPE65 promoter) or retinal but RPE aspecific (RHODOPSIN), glial (GFAP) or neural (SYNAPSIN and rat NSE) promoter. iRPE and fRPE cells were transduced using these vectors and the proportion of GFP-positive iRPE and fRPE cells was determined by FACS. In addition, the relative lentiviral genome copy number was quantified by qPCR. (b,c) EFS and R0.8 promoters showed a significant positive correlation between the percentage of GFP-positive cells (iRPE and fRPE together) and the relative lentiviral genome copy number whereas none of the aspecific promoters for RPE (d–g) did. R2: coefficient of determination of the linear regression. p: p-value from the F-test under the assumption of a null slope. P-value significance threshold: 0.05.

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