Figure 7 | Scientific Reports

Figure 7

From: Lentiviral mediated RPE65 gene transfer in healthy hiPSCs-derived retinal pigment epithelial cells markedly increased RPE65 mRNA, but modestly protein level

Figure 7

Lentivirus-mediated RPE65 gene augmentation expression in several RPE models, but poorly affects RPE65 protein level. iRPE, iCell RPE and fRPE cells were transduced with a lentivirus expressing RPE65 cDNA under the control of the R0.8 partial RPE65 promoter. Relative RPE65 mRNA and protein expressions were quantified  fourteen days later. (a) Comparison of normalized RPE65 mRNA expressions between untreated controls (Ctrl) and iRPE, iCell RPE or fRPE cells transduced with R0.8-RPE65 lentivirus at MOI 5. Dunn’s multiple comparisons test after Kruskal-Wallis test. P-value significance threshold: 0.05. Data are expressed as mean ± SD. (b) Comparison of RPE65 protein expression between untreated controls (Ctrl) and iRPE, iCell RPE or fRPE cells transduced with R0.8-RPE65 lentivirus at MOI 5 using unpaired t-tests. P-value significance threshold: 0.05. Data are expressed as mean ± SD. (c) Near infra-red imaging of RPE65 and GAPDH proteins on immunoblot. (d) Western blot densitometry analysis of RPE65 protein level normalized to GAPDH between untreated (Ctrl) and R0.8-RPE65 lentivirus-transduced iRPE, iCell RPE and fRPE cells (MOI 5). Mean ± SD. Unpaired t-test p-value significance threshold: 0.05.

Back to article page