Figure 2 | Scientific Reports

Figure 2

From: A species-specific miRNA participates in biomineralization by targeting CDS regions of Prisilkin-39 and ACCBP in Pinctada fucata

Figure 2

Gain-of-function assay of novel_miR_1 in vivo. Gain-of-function assay was conducted in vivo by injection of novel_miR_1 mimics. At 3d post injection, qRT-PCR was performed to evaluate the expression levels of novel_miR_1, putative target genes and several biomineralization-related genes in mantle tissues. (a–i) Relative expression level of novel_miR_1, Prisilkin-39, ACCBP, PfCHS1, PfMG11, N151, Nacrein, Shematrin-2, KRMP. The RNA-free water group was used as control. The results were analyzed by Student’s T test.

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