Figure 5 | Scientific Reports

Figure 5

From: Continuous Exposure to 1.7 GHz LTE Electromagnetic Fields Increases Intracellular Reactive Oxygen Species to Decrease Human Cell Proliferation and Induce Senescence

Figure 5

Continuous 1.7 GHz LTE RF-EMF exposure did not induce DNA damage and cellular apoptosis in ASCs and Huh7 cells. (A–D) ASCs and Huh7 cells were continuously exposed to 1.7 GHz LTE RF-EMF for 72 h at 1 SAR (A,B) or 2 SAR (C,D) and collected. Cells were lysed with lysis buffer and histones were extracted, which were separated by 8–15% SDS-polyacrylamide gel electrophoresis (PAGE) for western analyses. Western blots were performed with (A,C) anti γ-H2AX and anti-histone H3 antibodies, (B,D) anti-PARP and actin antibodies. Cells exposed to UV or treated with 3 μM doxorubicin (DOX) were used as positive controls for DNA damage and apoptosis. (A,C) Histone H3 and (B,D) β-actin from the same blot were used as a loading control. More than three independent replicates of all experiments were performed. The original full-length blots/gels were presented in the Supplementary Information.

Back to article page