Figure 1
A schematic diagram of pulmonary fixation and the structure of inflated lung. B. gargarizans exhibits scrofulas on the dorsal skin (A), and P. nigromaculatus has smooth dorsal skin (B). Schematic diagram of pulmonary fixation (C–H). The lung sample is placed at the end of a syringe filled with air and fixative (C). Air is injected into the lung (D). The syringe is placed in a downward position (E). The fixative is injected into the lung (F). The lung is soaked in fixative using two beakers (G). The samples was then removed for experiments (H). Surfaces of the inflated lungs of B. gargarizans (I-J”) fixed with Bouin’s fixative. Outer surface of the fixed lung: the fixed lung contains the pulmonary wall and is divided into many grids by primary (P) septa (I-I’). Inner surface of the fixed lung: the lung has many honeycomb faveoli that consist of primary, secondary (S), and tertiary (T) septa and part of the pulmonary wall (J-J”). I’ shows enlarged images of the boxes in I, and J’ and J” show enlarged images of the white boxes in J. Scale bars, I: 1.5 cm; I’: 0.4 cm; J-J”: 1.5 mm.
