Figure 5

Brown pigment production by strain JA2 in the presence of various inhibitors and substrates. (A) Effect of canonical melanin specific biosynthetic pathway inhibitors on brown pigment production under tryptophan-amended aerobic conditions. (B) Brown pigment production by strain JA2 grown in the presence of tryptophan and 5-hydroxytryptophan. Values are the mean ± standard deviation of three biological replicates. **P- value <0.005; ***P-val <0.0005 compared to Trp supplemented cultures, calculated using unpaired t-test. Trp, tryptophan; OH-trp, hydroxytryptophan; Glyph, glyphosate: KA, kojic acid; Quer, quercetin; Na-Az, sodium azide.