Table 1 Tryptophan derived metabolites detected in tryptophan-amended aerobic cultures of strain JA2.

From: Tryptophan, a non-canonical melanin precursor: New L-tryptophan based melanin production by Rubrivivax benzoatilyticus JA2

S. no.

Rt (min)

Generated Mol. formula

Exact Mass

m/z [M-]

Mass accuracy (ppm)

UV-visible Absorbance (nm)

Identification (Confirmed/ Tentative)

1

7.9

C11H12N2O3

220

219.0778

0.75

275

5-Hydroxytryptophan

2

10.9

C11H9NO4

207

206.0458

0.86

270, 280, 288

5,6-dihydroxyindole-3-acetic acid

3

14.3

C10H9NO3

191

190.0509

0.34

275, 300

5-hydroxyindole-3-acetic acid

4

15.5

C11H9NO4

219

218.0456

1.42

260, 292

5-Hydroxyindole-3- pyruvate

5

16.3

C9H9NO

147

146.0609

1.36

290

5-hydroxy-methyl-indole

  1. Metabolites in italics are confirmed by authentic standards.