Figure 5 | Scientific Reports

Figure 5

From: Identification of a periodontal pathogen and bihormonal cells in pancreatic islets of humans and a mouse model of periodontitis

Figure 5

Human pancreatic islets are positive for Pg/gingipain. (A) 9 out of 32 FFPE samples from human subjects (age >50 y) show the presence of Pg/gingipain in islets. Representative IF images. Scale bar represents 60μm. (B) 4 out of 13 frozen samples were positive for Pg 16S rRNA genes detected by qPCR. Y-axis: copies/5 FFPE slides. ***p < 0.001. (C) 3-D image from confocal microscopy of human pancreatic islet composed from Z-sections, showing Pg/gingipain (red) associated with β-cells. Scale bar represents 5 μm. (D) Orthogonal analysis of a β-cell confirming intra-nuclear localization of Pg/gingipain. a & b represent orthogonal projections. Red: Pg, Green: insulin, Blue: nuclei.

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