Figure 8

Human pancreatic bihormonal cells express Nkx6.1. (A) Representative images of bihormonal cells in DM subject expressing Nkx6.1 detected by IF microscopy. Scale bar represents 10 μm. For separate channel images of 40×, see Fig. S5. (B) 3-D image from confocal microscopy of a bihormonal cell expressing Nkx6.1. Scale bar represents 5 μm. (C) Orthogonal analysis of bihormonal cell staining for glucagon, Nkx6.1 and DAPI, (D) for insulin, Nkx6.1 and DAPI, and (E) for glucagon, insulin, Nkx6.1 and DAPI. a & b represent orthogonal projections. Yellow: glucagon, Red: insulin, Green: Nkx6.1, Blue: nuclei. (F) % of cells positive for Nkx6.1. A total of 76 α-, 118 β- and 30 bihormonal cells were counted. N = 4 DM subjects. Data show mean ± S.D. *p < 0.05, ****p < 0.0001.