Figure 4

The effect of DNA-LL37 complexes stimulation on the expression of IFN-α by monocytes of T1D and HC subjects. Representative flow cytograms showing the expression of intracellular IFN-α by monocytes. (a) Gating of monocytes based on FSC and SSC, (b) CD14 + monocytes, (c, d) Intracellular expression of IFN-α prior to after stimulation with DNA-LL37 complexes. (e) Frequency of IFN-α producing monocytes in T1D (n = 18) and HC (n = 10) subjects both prior to (MC) and after stimulation (Stim) with DNA-LL37 complexes (3 µg complex per 100 µL of media). (f) MFI of IFN-α producing monocytes in T1D and HC subjects before and after stimulation with DNA-LL37 complexes. Data is presented as mean (±SEM) frequency and one-way ANOVA followed by Tukey’s multiple comparison test was used to compare the means. P  <  0.05 was considered statistically significant. Relevant FMO tubes were used to set gates.