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Figure 1

From: Clearing method for 3-dimensional immunofluorescence of osteoarthritic subchondral human bone reveals peripheral cholinergic nerves

Figure 1

Human OA plug analysis. (A) Osteoarthritic human subchondral bone/cartilage plugs obtained from a macroscopically healthy zone of an osteoarthritic knee during arthroplasty for knee OA. (B) Histological analysis of a human subchondral bone/cartilage plug after paraformaldehyde fixation, decalcification, paraffin embedding and Safranin O/Fast Green staining for the analysis of all cartilage layers and subchondral bone. (C) Osteoarthritic human subchondral bone/cartilage plug after the 3DISCO clearing protocol.

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