Figure 3
From: GATA2 Regulates Constitutive PD-L1 and PD-L2 Expression in Brain Tumors
Identification of Novel CD274 and PDCD1LG2 cis-Regulatory Elements. (A) Cell surface expression of PD-L2 in brain tumor cells (grey line: isotype control; black line: PD-L2; dotted line: PD-L2 with IFN-γ stimulation). (B) CD274 promoter (Pr1) and enhancer (Pr2 and Pr3) regions cloned and assessed. (C) Normalized luciferase activity in cells with high and low endogenous PD-L1 expression using PD-L1.Pr1,.Pr2 and.Pr3 elements. (D) Schematic of PD-L1 enhancer region (Pr3) and truncated minimal region (Pr3M). (E) Luciferase assay of PD-L1.Pr3 (black) and.Pr3M (white) in PD-L1 high- and low-expressing cell lines. (F) PDCD1LG2 element (.Pr1 + 2) and truncated.Pr1 and.Pr2. (G) Luciferase assay of PD-L2.Pr1 + 2 (left) and PD-L2.Pr1 and.Pr2 (right) in PD-L2 high- and low-expressing cells. (H) PD-L2.Pr1 mutants lacking predicted STAT1 (ΔSTAT1), GATA2/3 (ΔGATA2/3), or both binding sites (ΔSTAT1 & ΔGATA2/3). (I) Luciferase assay of PD-L2.Pr1 truncations lacking STAT1 (ΔSTAT1) or GATA2/3 (ΔGATA2/3)(top) or also both (ΔSTAT1 & ΔGATA2/3)(bottom) in high and low expressing cell lines.
