Figure 4
From: GATA2 Regulates Constitutive PD-L1 and PD-L2 Expression in Brain Tumors
GATA2 Regulates PD-L1 and PD-L2 Expression. (A) PD-L1 (left panels) and PD-L2 (right panels) mRNA expression following GATA2 overexpression in LN340 (top panels) and LN464 (bottom panels). (B) PD-L1 (left panels) and PD-L2 (right panels) protein expression (grey line: antibody staining in control cells; black line: antibody staining in GATA2-transduced cells) following overexpression of GATA2 in LN340 (top) and LN464 (bottom). (C) Knockdown efficiency of GATA2 protein in IOMM-Lee cells by two different individual shRNA contructs measured by western blotting. ShLacZ is used as control vector (left); PD-L2 cell surface protein levels in IOMM-Lee cells transduced with control shRNA (grey line) or shRNA contructs targeting PD-L2 (black line, right panels). The blots were cropped from the same gel and the full blot image is included in the supplementary figure 5A. (D) Knockdown efficiency of GATA2 protein in AM38 (left); PD-L2 cell surface protein levels in AM38 cells transduced with control shRNA (grey line) or shRNA contructs targeting PD-L2 (black line, right panels). The blots were cropped from the same gel and the full blot image is included in the supplementary figure 5B.
