Figure 3

Hsa_circ_101237 sponges miR-490-3p to facilitate NSCLC development. (A) As shown in nuclear RNA fractionation and cytoplasmic experiments, hsa_circ_101237 could be mainly found in cytoplasm of H1299 cells and A549 cells. (B) The predicted binding site of miR-490-3p in hsa_circ_101237 3ʹUTR together with the mutated sites. (C,D) Co-transfection of miR-con or miR-490-3p and luciferase reporter vectors into H1299 cells and A549 cells. (E,F) RIP assay was conducted using the Ago2 and IgG antibody to immunoprecipitate. The expression of hsa_circ_0087862 and miR-490-3p was detected by qRT-PCR in H1299 cells and A549 cells. (G) qRT-PCR shew the expression of miR-490-3p in the NSCLC tissues and normal tissues nearby. (H) The expression correlation between miR-490-3p and Hsa_circ_101237 in 303 NSCLC tissues was determined by Pearson’s correlation analysis. *P < 0.05; **P < 0.01; ***P < 0.001.