Figure 3
Rate of acrosome-reacted sperm after selection. Sperm samples were dissolved in mHTF, and the flow rate was adjusted to approximately 400 events/s. The sperm were gated into fluorescence intensity of FITC-labelled PNA and collected in the collection reservoir. (A–E) We classified the sperm into three groups— AR-low, AR-middle and AR-high—according to intensity of FITC. After selection, the sperm were observed under fluorescence microscopy, and the number of sperm stained green were counted as acrosome-reacted sperm, AR (+). F, G) Fluorescence image of acrosome-intact sperm, AR ( − ). H, I) Fluorescence image of AR (+). J) The rate of acrosome-reacted sperm (%) was calculated as the number of AR (+) divided by the total number of AR (+) and AR (−). Values are given as the mean ± SD (n = 3). *p < 0.05 compared with AR-low.
