Figure 5
Effect of selection on sperm fertility and developmental ability. After selection, collected sperm suspensions were used as IVF medium. Oocytes were introduced into the IVF medium. Following IVF, the two-cell embryos were either cultured in KSOM/AA medium or transferred into the oviducts of pseudopregnant ICR females (10 embryos/oviduct). (A) The fertilisation rate was calculated as the number of two-cell embryos divided by the number of inseminated oocytes x 100. (B) The developmental rate was calculated as the number of embryos at several stages divided by the number of two-cell embryos x 100. (C) Two-cell embryos developed normally into live pups. The birth rate was calculated as the (number of live pups divided by the number of transferred two-cell embryos) x 100. Values are given as the mean ± SD (n = 3-5). *p < 0.05 compared with AR-low.
