Figure 3
From: Structural and catalytic analysis of two diverse uridine phosphorylases in Phytophthora capsici
Structural comparation and sequence alignment between uridine phosphorylases and purine nucleoside phosphorylases. (a) Comparation of structures among uridine phosphorylases. The hexamer-blocking insertions are shown in surface. PcUP1 in cyan; TbUP (PDB: 3bje), T. brucei uridine phosphorylase in yellow; HsUUP1 (PDB: 3euf), H. sapiens uridine phosphorylase1 in salmon; EcUP (PDB: 1tgy), E. coli uridine phosphorylase in magenta. (b) Structure-based sequence alignment of representative uridine phosphorylases together with purine nucleoside phosphorylases. Amino acid numbering and secondary structural elements of PcUP1 TBUP; HsUUP1; EcUP; TtPNP (PDB: 1odi), T. Thermophilus purine nucleoside phosphorylase; BsPNP (PDB: 4d8x), B. subtilis purine nucleoside phosphorylase are mapped at the top of the alignment. UP-specific inserts that block hexamer formation of PcUP1, TbUP and HsUUP1 are shaded cyan, yellow and salmon separately. The UP-specificity region is marked by the black box. The key substrate/product interacting residues are labeled “U” for uracil, “R” for ribose, “P” for phosphate. Structure-based sequence alignment was created using Clustal X Version 2.054 and ESPript 3.055.
