Figure 6
From: Structural and catalytic analysis of two diverse uridine phosphorylases in Phytophthora capsici
Relative activity of site-directed mutants of PcUP1 and PcUP2. Enzyme activity rates were compared to those of the native protein. Individual mutations reduced uridine phosphorylase activity by more than 50%. Mutation of residues that interact with the uracil or uracil-analogues exhibited lower enzyme activity than other mutants. Each assay was conducted in triplicate, from which the average ± s.d. was calculated.
