Figure 3 | Scientific Reports

Figure 3

From: 1,25(OH)2VitD3 supplementation enhances suppression of grass pollen-induced allergic asthma by subcutaneous and sublingual immunotherapy in a mouse model

Figure 3

The eosinophilic and cytokine response after VitD3-supplemented GP-SCIT. (A) Total cell counts in bronchoalveolar fluid (BALF) and lung single cell suspensions (Lung). (B) Differential cytospin cell counts in BALF and in (C) Lung. M, mononuclear cells; E, eosinophils; N, neutrophils. Absolute numbers are plotted in Box-and-whiskers plots (min-max). (D) BALF and lung eosinophils, both plotted as ratio of suppression (absolute EO/ average PC EO; mean ± SEM). (E) Levels of type 2 inflammatory cytokines IL4, IL5, IL13, regulatory cytokines IL10 and TGF-β1, and amphiregulin in pg/µg protein measured in lung tissue. Absolute values are expressed as mean ± SEM (n = 8). NC: Negative Control, PBS challenged; PC: Positive Control, GP challenged; PCD: PC with VitD3 in SCIT (10 ng), 100: 100kSQ SCIT, 100D: 100kSQ SCIT with 10 ng VitD3. *P < 0.05, **P < 0.01, ***P < 0.001 compared to PC or PCD respectively (100 vs PC and 100D vs PCD), unless otherwise specified.

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