Figure 2
THz wave effects on cellular actin filaments. (A) Live-cell imaging of actin filaments. The energy of the THz irradiation was 0, 80, 160, and 250 µJ/cm2. Cells imaged before (A, i-iv) and after 30-minute THz irradiation (A, v-viii). The red arrows indicate aggregated actin filaments. (B) Immunofluorescence images of the cell cortex stained with AlexaFluor 594-phalloidin. Images were observed using spinning-disk confocal microscopy. The right panels (v-viii) show the magnified images of the red squares in the left panels (i-iv), respectively. Red arrows indicate aggregated actin filaments. Arrowheads indicate filipodia fragments. Actin filaments were stained with SiR-actin and observed by fluorescence microscopy. The bar shows a scale of 10 µm. (C) Mean fluorescence intensity of phalloidin was analyzed using immunofluorescence images of the cell cortex. N > 51 cells/group. Asterisks indicate a statistically significant difference (**P < 0.01).
