Figure 2
From: CX3CL1 homo-oligomerization drives cell-to-cell adherence
Single–particle fluorescence analysis of CX3CL1. (A) Fluorescence of the membrane preparation of LCX3CL1 diluted in DOPC analyzed by TIRF. Bar 10 µm. (B) Examples of the fluorescence of particles tracked during one or two minutes. (C) Distribution of the fluorescence step amplitude according to the step number per particle. The grey bars represent the mean of the step amplitude in each case. (D) Distribution of the number of elementary fluorescence units per particle calculated after analysis of the fluorescence kinetic of 126 different particles. The Gaussian curve fits the data with an amplitude of 27.5, a mean of 4.3 and a standard deviation of 1.8.
