Figure 3
From: CX3CL1 homo-oligomerization drives cell-to-cell adherence
Diffusion rate of CX3CL1 transmembrane peptides analyzed by FRAP and FRAPP. (A) The fluorescence kinetics of Giant Unilamellar Vesicles containing either TM24-FITC (filled squares) or SC24-FITC (empty squares) were analyzed after bleaching by circles of various diameter (1, 2, 5 and 10 µm). The recovery constant time was reported versus the bleached area. Each measurement was the mean of triplicates. The slopes of the linear fit are 2.63 and 29.93 for TM24 and SCR24 respectively. (B)The diffusion rates were calculated based on the mean ± SEM of 12 measurements data used to give the (A). (C) The fluorescence kinetics of lipidic cubic “sponge” phase containing either TM24-FITC (filled squares) or SC24-FITC (empty squares) were analyzed after bleaching by interference pattern with various interfringe (20, 27, 46 and 58 µm). The recovery constant time was reported versus the interfringe distance. Each measurement is the mean of triplicates. The slopes of the linear fit are 252 ± 10 and 1093 ± 144 for TM24 and SCR24, respectively. (D) The diffusion rates were calculated based on the mean ± SEM of 12 measurements data used to give the (C). Note that the diffusion rate appeared dramatically higher in sponge phase since the fluorescent molecules could move in tridimensional milieu.
