Figure 8
From: CX3CL1 homo-oligomerization drives cell-to-cell adherence
Specific inhibition of the CX3CL1-dependent cell adherence by the peptide TM24. (A) Real time adherence of CHOCX3CR1 cells to L929 (dashed traces) or LCX3CL1 cells (solid traces) as assayed by the LigandTracer technique, in the presence of 5 µM TM24 (red traces), 5 µM SCR24 (blue traces) or none (black traces). The data were normalized using the control trace with LCX3CL1 cells in the absence of peptide (100% after 60 minutes). The curves are the mean of three independent experiments. (B) Specific adherence of CHOCX3CR1 cells to LCX3CL1 cells using data of (A). The data obtained with LCX3CL1 cells were subtracted from data obtained with L929 and normalized at 100% at the 60 minutes time. The bars in the right show the specific adherence after 60 minutes (mean of triplicates ± SD) in the presence of 5 µM TM24 (red) and 5 µM SCR24 (blue). (C) Real time binding of 100 nM of fluorescent CX3CL1 to coated CHOCX3CR1 cells using the LigandTracer technique, in the presence of 5 µM TM24 (red trace), 1 µM unstained CX3CL1 (dashed trace) or none (black trace). The data were normalized using the control trace without peptide (100% after 60 minutes). (D) Specific adherence of CHOCX3CR1 cells to LCX3CL1 cells after 60 minutes in the presence of various TM24 peptide concentrations and in the presence (filled squares) or in absence (empty squares) of 1 µg/ml of anti-CX3CL1 antibody. The data were calculated and normalized as in (B). Experiments are performed in duplicate except for the 1 µM TM24 concentration done in triplicates (mean ± SD).
