Table 2 Daily Growth Rate (DGR %), Agar yield (%), Sulfate (%), 3,6-Anhydrogalactose (3,6-AG %), Proteins (%) and R-phycoerythrin (R-PE mg g−1fw) obtained from Gracilaria gracilis samples cultured at different depths in two sites (Bizerte Lagoon and Bizerte Bay, Northeast Tunisia) analyzed by ANOVA at 95% confidence level.
Factors | SS | df | MS | F | p |
|---|---|---|---|---|---|
DGR (%) | |||||
Intercept | 154.07 | 1 | 154.07 | 1557.88 | <0.05 |
Site | 1.22 | 1 | 1.22 | 12.36 | <0.05 |
Depth | 7.85 | 14 | 0.56 | 5.67 | <0.05 |
Site*Depth | 10.24 | 14 | 0.73 | 7.39 | <0.05 |
Error | 5.93 | 60 | 0.10 | ||
Agar (%) | |||||
Intercept | 40636.38 | 1 | 40636.38 | 6314.463 | <0.05 |
Site | 382.75 | 1 | 382.75 | 59.475 | <0.05 |
Depth | 110.93 | 14 | 7.92 | 1.231 | >0.05 |
Site*Depth | 58.34 | 14 | 4.17 | 0.648 | >0.05 |
Error | 386.13 | 60 | 6.44 | ||
Sulfate (%) | |||||
Intercept | 4932.622 | 1 | 4932.622 | 3507.418 | <0.05 |
Site | 176.084 | 1 | 176.084 | 125.207 | <0.05 |
Depth | 54.125 | 14 | 3.866 | 2.749 | <0.05 |
Site*Depth | 74.098 | 14 | 5.293 | 3.763 | <0.05 |
Error | 84.380 | 60 | 1.406 | ||
3,6-AG(%) | |||||
Intercept | 97159.40 | 1 | 97159.40 | 36228.44 | <0.05 |
Site | 6518.91 | 1 | 6518.91 | 2430.75 | <0.05 |
Depth | 853.10 | 14 | 60.94 | 22.72 | <0.05 |
Site*Depth | 1196.31 | 14 | 85.45 | 31.86 | <0.05 |
Error | 160.91 | 60 | 2.68 | ||
Proteins(%) | |||||
Intercept | 41150.41 | 1 | 41150.41 | 979.3834 | <0.05 |
Site | 36.62 | 1 | 36.62 | 0.8715 | >0.05 |
Depth | 976.25 | 14 | 69.73 | 1.6596 | >0.05 |
Site*Depth | 661.06 | 14 | 47.22 | 1.1238 | >0.05 |
Error | 2521.00 | 60 | 42.02 | ||
R-PE(mgg−1fw) | |||||
Intercept | 31.92 | 1 | 31.92 | 535.76 | <0.05 |
Site | 6.30 | 1 | 6.30 | 105.83 | <0.05 |
Depth | 1.15 | 14 | 0.08 | 1.38 | >0.05 |
Site*Depth | 0.23 | 14 | 0.02 | 0.27 | >0.05 |
Error | 3.57 | 60 | 0.06 | ||