Table 1 List of all 5 treatments used for the nHA microbiome experiment with replicate number and added solutions. Additional growth replicates were included during microbiome experiment to assess growth of plants under experimental conditions.

From: A practical assessment of nano-phosphate on soybean (Glycine max) growth and microbiome establishment

 

Treatment

Final Concentration

Phosphorus Concentration**

Microbiome Replicates

Growth Replicates

Added Solution

A

Control

—

—

20

5 + 3 extra

Water

B

−P Control*

—

—

20

5 + 3 extra

KNO3 & Hoagland’s***

C

HA Bulk Control (50 mg/kg)

50 ppm (4.3 mg HA)

9.25 ppm P

20

5 + 3 extra

KNO3 & Hoagland’s***

D

nHA (50 mg/kg)

50 ppm (4.3 mg nHA)

9.25 ppm P

20

5 + 3 extra

KNO3 & Hoagland’s***

E

nHA (100 mg/kg)

100 ppm (8.6 mg nHA)

18.5 ppm P

20

5 + 3 extra

KNO3 & Hoagland’s***

  1. *P control contains no added Pi to the soil either at time of planting or in fertilizing solution.
  2. **Concentration of P based on equivalent moles of P added in either 50 or 100 ppm of HA or nHA in 86 g of soil.
  3. ***Hoagland’s solution contains no N or P.
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