Figure 1
Cell classes in human retina. (a) (Top) Sketch of a human eye showing positions of retina and fovea. (Bottom) Sketch of a flat mounted retina showing foveal and peripheral regions. Circle in center is position at which optic nerve exits the eye. (b) Sketch of a peripheral retinal section showing its major cell classes—photoreceptors (PRs), horizontal cells (HCs), bipolar cells (BCs), amacrine cells (ACs), retinal ganglion cells (RGCs) and Müller glia (MG), outer and inner plexiform (synaptic) layers (OPL and IPL), outer and inner nuclear layers (ONL and INL), and ganglion cell layer (GCL). (c) scRNA-seq workflow. Foveal cells were dissociated from <1.5 mm-diameter punches and collected without further processing. Peripheral cells were dissociated from all four quadrants of peripheral retinas, and depleted of rods (CD73+) or enriched for RGCs (CD90+) with magnetic columns before processing. (d) Expression patterns of class-specific markers (rows) in individual cells (columns). Cells are grouped by their classes (color bars, top). Plot shows data from a maximum of 500 randomly selected cells per class.
