Table 1 Amount of inserted LacY and XylE in each lipid composition.
From: Cell-free expression tools to study co-translational folding of alpha helical membrane transporters
Lipid composition | Amount of inserted protein (% of total protein expressed) | |
|---|---|---|
LacY | XylE | |
100 DMPC | 17.0 ± 2.7 | 39.0 ± 7.0 |
100 DOPC | 25.9 ± 1.6 | 32.8 ± 0.6 |
50:50 DOPC:DOPE | 32.7 ± 1.7 | 44.9 ± 1.6 |
50:50 DOPC:DOPG | 34.8 ± 1.4 | 32.3 ± 1.5 |
50:50 DOPE:DOPG | 34.3 ± 2.5 | 49.1 ± 1.0 |
100 DOPG | 44.7 ± 1.0 | 58.7 ± 2.4 |
75:25 DOPE:DOPG | 37.7 ± 2.5 | 35.5 ± 4.0 |
40:40:20 DOPC:DOPE:DOPG | 34.5 ± 2.4 | 33.3 ± 1.4 |
25:50:25 DOPC:DOPE:DOPG | 29.4 ± 1.7 | 38.1 ± 2.3 |
