Table 1 Kinetic rate constants, dissociation constants.

From: The trimer to monomer transition of Tumor Necrosis Factor-Alpha is a dynamic process that is significantly altered by therapeutic antibodies

Immobilized Ligand

Analyte in Solution

kON (106 M−1s−1)

kOFF (10−5 s−1)

KD (pM)

TNF-α

Adalimumab Fab

2.82 (0.10)

8.52 (0.28)

30.2 (1.5)

Adalimumab Fab

TNF-α

6.73 (0.03)

8.62 (0.23)

12.8 (0.3)

TNF-α

Adalimumab

8.22 (0.15)

7.96 (0.24)

9.7 (0.3)

Adalimumab

TNF-α

7.86 (0.07)

4.60 (1.19)a

5.9 (1.5)

  1. aError is standard error of mean of individually fitted kOFF.
  2. A comparison to literature values is drawn in the supplementary section (Table S1).
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