Figure 2

Oxygen consumption, ATP synthesis and P/O value in the presence of pyruvate/malate or succinate. Panel A: Oxygen consumption after stimulation with pyruvate/malate (P/M) (light grey column) or succinate (dark gray) in MNCs isolated from young healthy subjects (CTRL 8–20 yrs, n = 18), adult healthy subjects (CTRL 21–60 yrs; n = 18), elderly healthy subjects (CTRL 61–86 yrs, n = 25) and MDS patients with iron overload (n = 22). Panel B: aerobic ATP synthesis after stimulation with P/M (light grey column) or succinate (dark gray) in MNCs isolated from young healthy subjects (CTRL 8–20 yrs, n = 18), adult healthy subjects (CTRL 21–60 yrs; n = 18), elderly healthy subjects (CTRL 61–86 yrs, n = 25) and MDS patients with iron overload (n = 22). Panel C: P/O ratio, as marker of OxPhos efficiency, in MNCs isolated from young healthy subjects (CTRL 8–20 yrs, n = 18), adult healthy subjects (CTRL 21–60 yrs; n = 18), elderly healthy subjects (CTRL 61–86 yrs, n = 25) and MDS patients with iron overload (n = 22). For each Panel, data are expressed as mean ± SEM. Data are analyzed by one-way ANOVA followed by Tukey’s multiple comparison test. *, **, ***, **** indicate a significant difference for p < 0.05, p < 0.01, p < 0.001, p < 0.0001, respectively, between MDS sample and the healthy controls.