Figure 2

Aβ deposits and cell death in choroid plexus from AD patients. (a) Representative micrographs showing Aβ deposits in choroid plexus from early and advanced AD cases compared with healthy donors. Scale bar = 20 μm. (b) Quantified levels of Aβ burden in choroid plexus from healthy donors compared with AD subjects measured by ELISA (Cell Death Detection ELISAPLUS kit, Roche). Scatter plot reveals a progressive increase in Aβ levels in choroid plexus samples from AD subjects compared with control group (*p < 0.05; control n = 7; AD I-II n = 12; AD III-IV n = 11; AD V-VI n = 8). In all cases, each individual value is shown along with the mean ± SEM per group. (c) Cell death analysis in choroid plexus from control and AD patients was measured using Cell Death Detection ELISAPLUS kit. Scatter plot shows a significant increase in cell death in choroid plexus from advanced AD patients (Braak stages V–VI) compared with control subjects (*p < 0.05; control n = 7; AD I-II n = 11; AD III-IV n = 11; AD V-VI n = 8). In all cases, each individual value is shown along with the mean ± SEM per group. (d) Representative micrographs showing annexin A5 immunostaining in choroid plexus from early and advanced AD cases compared with healthy donors. Scale bar = 20 μm.