Table 3 Primer sets used to detect progeny following a cross of transgenic and weedy rice.
Primer set | Sequence (5′ to 3′) | Amplified size (bp) |
|---|---|---|
Specific to AtCYP78A7 cassette | ||
AtCYP78A7- F | GAT GTT TCC ACC CTA GGC TA | 787 |
AtCYP78A7- R | TTT TCT TGA CGA GGG TTC TA | Â |
Positive control | ||
RBE4-F | GTT TTA GTT GGG TGA AAG CGG TT | 105 |
RBE4-R | CCT GTT AGT TCT TCC AAT GCC CTT A | Â |
Zygosity determination | ||
AtCYP78A7- LBcfm R | AAA AGA TGA GGC CTT GAC TG | 440 |
AtCYP78A7-insertion-F1 | CGT GTC TGC GTC CAA GTA CA | Â |
