Figure 6
LCB or LCB-RM promotes dose- and time-dependent degradation of IκBα in differentiated 3T3-L1 adipocytes. (A), a representative Western blot data set shows the kinetic degradation of IκBα protein. Differentiated 3T3-L1 adipocytes were treated with LCB at the ratio of 1:150 cells:LCB or with 1% LCB-RM, and the cells were collected at 30, 60 and 120 minutes for Western blot analysis using anti-IκBα and anti-β-actin antibodies. LPS at 100 ng/mL concentration was used as a positive control. The IκBα:β-actin protein intensity ratio at each time point was calculated and expressed as the relative ratio in respect to the value from the untreated at zero minute, as shown in the chart. (B), a representative Western blot data set shows dose-dependent effect of LCB or LCB-RM on IκBα degradation. Differentiated 3T3-L1 adipocytes treated with an increasing amount of LCB or LCB-RM for 30 minutes were collected for Western blot analysis using anti-IκBα and anti-β-actin antibodies. The intensity ratio of IκBα:β-actin proteins were calculated and shown in the chart as described in (A). UC and UC-RM are abbreviated for unconjugated beads and unconjugated bead-treated conditioned medium from RAW 264.7 macrophages (UC-RM).
