Table 2 Chromatographic and spectroscopic properties of Orfelia and Neoditomiya hot extracts and partially isolated luciferins.
Fraction | Rf* solv. I | Rf solv. II | λabs (nm) | λEX (nm) | λFL (nm)** |
|---|---|---|---|---|---|
Orfelia hot extract | — | — | 362 | 457 | |
Neoditomiya hot extract | — | — | 362 | 458 | |
Hot extract air oxidized | — | — | 362 | 459 | |
TLC-eluted origin | 0 | 0 | 257/347 | 360 | 442 |
TLC-eluted origin (oxidized) | 251/350 | 360 | 460 | ||
TLC-eluted band 1 | 0.66 | 0.74 | — | 360 | 443 |
TLC-eluted band 2 | 0.77 | 0.8 | — | 360 | 447/~540 |
Qcolumn-Eluted Luciferin | — | — | 254/365 | 356 | 447 |
Oxidized Qcolumn-Eluted luciferin | — | — | 362 | 457 |
