Figure 3 | Scientific Reports

Figure 3

From: Comparative targeting analysis of KLF1, BCL11A, and HBG1/2 in CD34+ HSPCs by CRISPR/Cas9 for the induction of fetal hemoglobin

Figure 3

RNA-seq analysis. (a) Number of genes down- and up-regulated after targeting KLF1, BCL11A, and HBG1/2. (b) Number of common dysregulated genes for the three individual experiments. (c) Heatmap showing the Log2 fold change of the common differentially expressed genes after gene editing in KLF1, BCL11A, and HBG1/2. R1, R2, and R3 represent the three performed replicates. Dysregulated genes are depicted in a blue-to-red color gradient, where down-regulated genes are shown in blue and up-regulated genes in red. (d) Number of reads indicated as clusters per million (cpm) for hemoglobin genes in edited samples relative to the control sample. (e,f) Cpm values for the most relevant genes regulated by KLF1 (e) and BCL11A (f).

Back to article page