Figure 3
From: Transcriptional control of the gonococcal ompA gene by the MisR/MisS two-component regulatory system
Mapping of transcriptional start sites in the ompA promoter by primer extension. (a) Electropherograms of fluorescently (HEX) labeled primer extension products for FA19, RNase treated FA19 and misR-null (JK100) and the corresponding sequence ladder. The nucleotides shown below the sequence ladder correspond to the promoter region of ompA. Primer extension products were analyzed using Applied Biosystems GeneMapper Software version 4.0 (https://www.thermofisher.com/order/catalog/product/4440915#/4440915). (b) Sequence of the ompA promoter region with predicted MisR-binding sites. Red boxes indicate predicted MisR-binding sites using the PRODORIC online tool. The −10, −35 and Shine-Dalgarno promoter elements and the transcriptional start site (TSS) are indicated with text and underlined. The ATG ompA start codon is highlighted in purple. MisR binding site consensus sequence IUPAC code: KWWWTGTAARGNNWH where K = G/T, W = A/T, R = A/G, H = A/T/C, and N = any nucleotide. The nucleotide sequence within the S1 site that was mutated in TruncΔS1 is shown in italics.
