Figure 5

Correlation of imaging versus histology results. (A) Radioactivity of the muscle tissue samples from the injected limb (black bars in the histogram), and other tissue samples (grey bars), expressed as a percentage of the injected dose, and normalised by the volume of the sample. Each bar of the histogram represents a sample from one dog. (B) Correlation plot between the radioactivity of the muscle samples and the number of DiI⁺ cells. The results show that there is correlation between the images and radioactivity. (C) Correlation plot between the radioactivity of the samples from non-muscle tissues, and the number of DiI⁺ cells, showing no correlation. The regression straight line obtained for muscles was superimposed on this graph illustrating the fact that radioactivity in the liver was not associated with the presence of DiI⁺ cells, and that DiI⁺ cells in the lung did not correlate with the presence of radioactivity. In this latter tissue, the DiI⁺ signal is thus probably not arising from transplanted cells, but rather result from a transfer from dead cells to host cells. (D) Image from a muscle sample showing DiI⁺ cells (DiI in orange) (E) Image from a lung sample showing DiI⁺ cells (F) Image from a liver sample showing no DiI⁺ cell. Abbreviations: Tibialis cr.: tibialis cranialis; Gastroc.: gastrocnemius; IO: interosseous; VM: vastus medius; BF: biceps femoris; Sp: spleen: Ki: kidney.