Figure 3
(a) STXM absorption image of a 7 µm thick soybean root section where the XRF maps were collected (80 µm × 80 µm, 100 × 100 pixels, 40 ms dwell time/pixel, scale bar = 20 µm). (b) Resulting dynamic XRF scan where the point indicated in black in panel (c) where acquired with 1 s acquisition time, while the one in green with 7 s, all normalised to 1 s acquisition time. (c) According to the pre-set XRF threshold the points in black were acquired at 1 s and the ones in green at 7 s. Full XRF map acquired with 1 s/pixel (d) and 7 s/pixel (e). In this specific case Panels b, d, and e show Na XRF signal collected at 1.95 keV over an area of 80 µm × 80 µm, with 800 nm step size.
