Table 1 List of primers.
From: Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora
Primer ID | PCR type | 5’−3’ Sequence | Tm (°C) | Amplicon size (bp) |
|---|---|---|---|---|
EaGLWamide ORF F | RT-PCR | AGGGGAATGTCGCTACGT | 45 | 1149 |
EaGLWamide ORF R | RT-PCR | GCGAATGTTTATTTGCTT | 45 | — |
EaGLWamide 5′ | 5′ RACE | GTACTGCTCGCCCCCAGAGTCCTGGTGG | 68 | 643 |
EaGLWamide 5′ nested | 5′ RACE | CATCTTCCGTTTCTTCGTCCTCTTCGCC | 62 | 568 |
EaGLWamide 3′ | 3′ RACE | CCACAGAAGGTTGACGAATCGGACGAAG | 62 | 769 |
EaGLWamide 3′ nested | 3′ RACE | GGCGAAGAGGACGAAGAAACGGAAGATG | 62 | 691 |
EaGLWamide F1 | Quantitative RT-PCR | GTTGACGAATCGGACGAAGA | 60 | 157 |
EaGLWamide R1 | Quantitative RT-PCR | AGAGTCCTGGTGGTGCTCTGAT | 60 | — |
β-actin F | Quantitative RT-PCR | CGCCTTCCTTGGAATGGAATCCTCT | 60 | 151 |
β-actin R | Quantitative RT-PCR | CTGCATCCTGTCAGCGATTCCAGG | 60 | — |
