Figure 6
(A–D) TA formation on the test zone of the strip. The moment of sample solution front reaching test zone is set as T = 0 s. (E,F) AuNPs diffusion images and (G) dynamics in the lateral flow assays with direct sampling method and test-zone pre-enrichment method. Only buffer solution was loaded after conjugate pad addition and the volumes of buffer solution are both 50 μL in two methods. The moment of buffer solution front reaching NC membrane is set as T = 0 s after conjugate pad addition, and the LFA is developed about 10 min. The integrated densities of gray in blue box were analyzed by ImageJ to investigate the diffusion of AuNPs. (H–K) TAP formation statistic on the test zone of the strip after DP-AuNPs loading for 10 min.
