Table 3 Gene-specific primers used in different reactions.
From: Identification and expression of DoCCaMK during Sebacina sp. symbiosis of Dendrobium officinale
Primers | Forward | Reverse |
|---|---|---|
cDNA synthesis of CCaMK | GACCATCTAGTTTCAAAACCC | CATTCATTACAAAGCGTGC |
Cloning of DoCCaMK | GSP1: CATCTTCCACAATCCGTCTCATCACC GSP2: CTAAGCCGCAAGCTATCTGGCGCAC | GSP3: GGGCAGAAACCTCCAACATTGAAGCA GSP4: GATTCTGGTGATGAGACGGATTGTGG |
qRT-PCR | CACTCAAAAGGTTAGGGTTCAT | TCTCATTAGTCAGCAAAGCATC |
Construction of CCaMK-GFP fusion expression vector | GTCGACATGTCGAGCCTGGAGAATAGAAAG | GGATCCGTTAGGGCGCAGTGTGGAGA |
CCaMK-TE | GGATCCATGTCGAGCCTGGAGAATAGAAAG | GTCGACCTAGTTAGGGCGCAGTGTGGAG |
