Figure 6
The effect of TGF-β1 on SPOCK1 expression in gingival keratinocytes and fibroblasts. Gene and protein expression levels of SPOCK1 in MOE1a and primary human gingival fibroblasts were measured by RT-PCR and western blotting, respectively. (a) Relative SPOCK1 mRNA and protein expression levels in MOE1a cells stimulated with 5 ng/mL TGF-β1 for 2 and 3 days. (b) Relative SPOCK1 mRNA and protein expression levels in primary HGF stimulated with 5 ng/mL TGF-β1 for 2 and 3 days. Immunoblot data are representative of three independent experiments with similar results. Gene expression analyses were performed at least three times with triplicate samples. Data are presented as mean ± SEM; NS; not significant, **P < 0.01, ***P < 0.001 compared with unstimulated controls by analysis of variance with Tukey’s test (n = 3).
