Figure 8
Time-course analysis of the formation and uptake of amino acids by different UPEC strains. Metabolite levels standardized using the control as a baseline, consisting of sterile urine with 5 µL of LB media to account for potential matrix effect. A linear mixed-effect model with repeated measures was used to calculate statistical significance. Tukey post-hoc test was used to determine individual differences (Tables S1 and S2). The horizontal significance line and p-value correspond to the main effect of Time over the concentration of each metabolite. Vertical significance corresponds to the comparison of each strain vs the control after 24 h. Data are presented as means of 3 independent experiments ± SEM. (**p < 0.01, *****p < 0.0001).
