Figure 3 | Scientific Reports

Figure 3

From: Recombinant Bri3 BRICHOS domain is a molecular chaperone with effect against amyloid formation and non-fibrillar protein aggregation

Figure 3

Rh Bri3 BRICHOS suppresses thermally induced non-fibrillar aggregation of model substrates. (a) Aggregation of 600 nM citrate synthase (CS) alone (dark brown) and in the presence of different molar ratios of rh Bri3 BRICHOS (shades of red) at 45 °C. Representative data for triplicates shown as mean ± SD. (b) The final intensity of CS aggregation alone (black) or in the presence of different molar ratios of rh Bri3 (red) or Bri2 BRICHOS (blue). (c) Aggregation of 3 µM rhodanese alone (dark brown) or in the presence of different molar ratios of rh Bri3 BRICHOS (shades of red) at 45 °C presented as mean ± SD of triplicates. (d) The final intensity of rhodanese aggregation alone (black) or in the presence of different molar ratios of rh Bri3 (red) and Bri2 BRICHOS (blue). The data in panels (b) and (d) are baseline corrected and normalised to the mean of CS or rhodanese maximum intensity, respectively, and presented as mean ± SD of 3 experiments performed in triplicates.

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